By marcel florkin
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Extra info for Comparative Biochemistry. A Comprehensive Treatise
From James and James (86). progressive stages of development. The arrows indicate the approximate stage at which the substances named are exhausted. In the first phase, a rapid development of the embryo occurs at the expense of sucrose and raffinose stored in the embryo. In phase 2, embryonic reserves are exhausted and endospermic starch is converted to dextrins which in turn form sucrose. Dextrins and sucrose reach maximal concentrations when respiration is at its peak. Sucrose is considered to be the immediate substrate of respiration in the embryo; when added exogenously, this substance stimulates respiration about threefold throughout the germina tion process.
The total radioactivity of the precursor S -methionine pool is constantly diminishing, and fluctuations in the concentration of pool methionine (and hence its specific radioactivity) may also be taking place, owing to the liberation of unlabeled methionine from endogenous protein reserves, for example. Again, information is needed on the specific radioactivity of intracellular methionine at the develop mental stages examined. In view of the above reservations concerning data on apparent alterations in the rate of protein synthesis during development, a recent study using mitochondria isolated from sea urchin eggs is of much interest.
04 Total Ash (%) 1. BIOCHEMISTRY OF MORPHOGENESIS 35 Table X I summarizes the redistribution of reserve materials in the germinating castor bean (88). As germination progresses, a striking decrease in fat content occurs accompanied by an increase in total reducing sugar. Q. value as a function of the stage of germination is given for the castor bean in Fig. 13. ) FIG. 1 3 . Respiratory quotients during germination in the castor bean. From Daggs and Halcro-Wardlaw (89). germinated in darkness in order to ensure their complete dependence upon endogenous reserves.
Comparative Biochemistry. A Comprehensive Treatise by marcel florkin